Figure 5 | Scientific Reports

Figure 5

From: Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice

Figure 5

Cre/loxP-mediated gene loading to the 10MAC2 and 10MAC3. (A,D) Strategy for the site-specific insertion of the EGFP and tdTomato genes into the 10MAC2 and 10MAC3 vectors, respectively, using the Cre/loxP-mediated system in CHO cells. (B) Transfection yielded GFP-expressing HAT-resistant transfectants from the CHO (10MAC2) cells. Phase-contrast (top panel) and fluorescence (bottom panel) micrographs are shown. Scale bar: 100 μm. (C) FISH analysis of the GFP-10MAC2 in the CHO (GFP-10MAC2) cells using the digoxigenin-labeled mouse Cot-1 DNA (red) and the biotin-labeled EGFP gene (green). The arrow indicates the GFP-10MAC2 vector, and the inset shows an enlarged image of the GFP-10MAC2 vector. (E) Transfection yielded tdTomato-expressing HAT-resistant transfectants from the CHO (10MAC3) cells. Phase-contrast (top panel) EGFP (middle panel) and tdTomato (bottom panel) micrographs are shown. Scale bar: 100 μm. (F) FISH analysis of the tdTomato-10MAC3 in the CHO (tdTomato-10MAC3) cells using the digoxigenin-labeled mouse Cot-1 DNA (red) and the biotin-labeled tdTomato gene (green). The arrow indicates the tdTomato-10MAC3 vector, and the inset shows an enlarged image of the tdTomato-10MAC3 vector.

Back to article page