Figure 6
Generation of Tc mice carrying 10MAC1 and its stability in the tissues. (A) Phase-contrast (top panel) and fluorescence (bottom panel) micrographs of mouse ES hybrids carrying 10MAC1 are shown. Scale Bar: 100 μm. (B) FISH analysis of the mouse ES hybrids carrying 10MAC1 using the digoxigenin-labeled mouse minor satellite DNA (red) and the biotin-labeled pVGNLH1 (green). Inset shows an enlarged image of the 10MAC1. (C) Phase-contrast (top panel) and fluorescence (bottom panel) micrographs of wild-type (right) and Tc mice (left) are shown. (D) Representative FISH image of metaphase chromosome spreads of lymphocytes in blood from Tc mice using the digoxigenin-labeled mouse minor satellite DNA (red). Inset shows an enlarged image of the 10MAC1. (E) GFP images of different tissues from Tc mice carrying the 10MAC1. GFP expression indicates the presence of 10MAC1. Exposure times for each GFP image of small intestine, brain, kidney, heart, liver, lung, skeletal muscle, spleen, testis, and thymus were 100, 500, 200, 100, 400, 700, 100, 400, 200 and 200 ms, respectively. Scale bar: 3 mm. (F) Percentage of GFP-expressing lymphocyte subsets from blood, bone marrow and spleen.
