Table 4 Purine metabolism gene panel RT-qPCR results. mRNA levels in the livers of 19 week old prenatally Dex-exposed offspring relative to saline control. Fold changes in gene expression were calculated by relative quantification (ΔΔCt) of RT-qPCR threshold cycles (Ct) as per Livak and Schmittgen²⁸ using mean Ct values of housekeeping genes GAPDH and Rpl32. Data is expressed as mean fold change of Dex relative to the saline group ± SEM (n = 8). Unpaired t-test: Statistical significance between groups is shown by: * p < 0.05, ** p < 0.01.

From: Untargeted metabolomics reveals sex-specific differences in lipid metabolism of adult rats exposed to dexamethasone in utero