Table 5 Glutathione metabolism gene panel RT-qPCR results. mRNA levels in the livers of 19 week old prenatally Dex-exposed offspring relative to saline control. Fold changes in gene expression were calculated by relative quantification (ΔΔCt) of RT-qPCR threshold cycles (Ct) as per Livak and Schmittgen28 using mean Ct values of housekeeping genes GAPDH and Rpl32. Data is expressed as mean fold change of Dex relative to the saline group ± SEM (n = 8). Unpaired t-test: Statistical significance between groups is shown by: * p < 0.05.
Gene | Description | Sex | Fold change ± SEM |
|---|---|---|---|
GSR | Glutathione reductase | Male Female | 1.19 ± 0.30 1.40 ± 0.22 * |
GPX1 | Glutathione Peroxidase | Male Female | 0.98 ± 0.21 0.97 ± 0.18 |
GGT1 | Glutathione hydrolase | Male Female | 1.25 ± 0.18 0.63 ± 0.31 |
SMS | Spermine synthase | Male Female | 0.96 ± 0.10 1.02 ± 0.12 |
CAT | Catalase | Male Female | 0.99 ± 0.07 1.08 ± 0.08 |
SOD1 | Superoxide dismutase 1 | Male Female | 1.05 ± 0.23 0.73 ± 0.20 * |
SOD2 | Superoxide dismutase 2 | Male Female | 1.05 ± 0.18 0.84 ± 0.14 |
