Table 6 Circadian rhythm gene panel RT-qPCR results. mRNA levels in the livers of 19 week old prenatally Dex-exposed offspring relative to saline control. Fold changes in gene expression were calculated by relative quantification (ΔΔCt) of RT-qPCR threshold cycles (Ct) as per Livak and Schmittgen28 using mean Ct values of housekeeping genes GAPDH and Rpl32. Data is expressed as mean fold change of Dex relative to the saline group ± SEM (n = 8). Unpaired t-test: Statistical significance between groups is shown by: * p < 0.05.
Gene | Description | Sex | Fold change ± SEM |
|---|---|---|---|
CLOCK | Clock circadian regulator | Male Female | 0.88 ± 0.10 0.94 ± 0.19 |
BMAL1 | Brain and muscle ARNT like protein 1 | Male Female | 0.65 ± 0.42 0.66 ± 0.65 |
NPAS2 | Neuronal domain PAS protein 2 | Male Female | 0.75 ± 0.49 0.83 ± 0.56 |
PER1 | Period circadian protein homolog 1 | Male Female | 0.95 ± 0.38 1.59 ± 0.43 |
PER2 | Period circadian protein homolog 2 | Male Female | 1.56 ± 0.21 * 0.93 ± 0.51 |
PER3 | Period circadian protein homolog 3 | Male Female | 1.28 ± 0.79 1.23 ± 1.00 |
CRY1 | Cryptochrome circadian clock 1 | Male Female | 0.83 ± 0.46 1.26 ± 0.26 |
CRY2 | Cryptochrome circadian clock 2 | Male Female | 1.09 ± 0.15 1.12 ± 0.23 |
REV-ERBA | Nuclear Receptor Subfamily 1 Group D Member 1 | Male Female | 0.70 ± 1.05 0.66 ± 0.78 |
METTL3 | N6-adenosine-methyltransferase 70 kDa subunit | Male Female | 1.10 ± 0.14 0.80 ± 0.17 |
FBXL3 | F-box and leucine rich repeat protein 3 | Male Female | 1.04 ± 0.16 0.92 ± 0.19 |
CSNK1D | Casein kinase I isoform delta | Male Female | 1.01 ± 0.10 0.92 ± 0.11 |
CSNK1E | Casein kinase I isoform epsilon | Male Female | 0.78 ± 0.20 1.12 ± 0.44 |
