Figure 3
Potentiation of irradiation effect by MLN8237. Normalized viability values ± standard errors of mean are shown (N = 4). MLN8237 (final concentration of 25 nM) was added onto U-251 MG (a) or T98-G (b) cells either 24 h prior to irradiation (pre-treatment) or 2 h following the irradiation (post-treatment). Following irradiation, the cells were grown for a further 48 h. The asterisks above the top arrows in the graphs indicate the significance of grouped comparisons of each treatment condition to the PBS-treated cells on the same plate (one-way ANOVA; *** P < 0.001; ** P < 0.01). The asterisks above the middle set of arrows in the graphs indicate pairwise comparisons for the pre-treated and post-treated columns (unpaired t-test; *** P < 0.001; ** P < 0.01; * P < 0.05; ns, not significant). The grids below the graphs show pairwise comparisons (the compared columns are indicated with X) for the pre-treated or post-treated cells under different irradiation conditions (unpaired t-test; *** P < 0.001; ** P < 0.01; * P < 0.05; ns, not significant).
