Figure 4
Confirmation of synergistic effect of lomustine and azacytidine in GB cell lines as determined by IF assay. (a) Effect of individual compounds and their mixture on the cell number following 48 h treatment. The graph shows the number of nuclei normalized to the PBS control for each individual experiment ± standard errors of mean (N = 3). The asterisks above the arrows in the graphs indicate the significance of grouped comparison of each treatment condition to the mixture-treated cells in the same cell line (one-way ANOVA; *** P < 0.001; ** P < 0.01; * P < 0.05; ns, not significant). (b) Effect of individual compounds and their mixtures on the γH2AX levels in the nucleus following 48 h treatment. The graph shows mean average γH2AX signal intensities ± standard errors of mean (N = 3). The asterisks above the arrows in the graphs indicate the significance of grouped comparison of each treatment condition to the PBS control in the same cell line (one-way ANOVA; ***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant). The asterisks above the middle set of arrows in the graphs indicate pairwise comparisons for treatments with lomustine vs mixture (unpaired t-test; ns, not significant).
