Figure 8
From: Metabolomic credentialing of murine carcinogen-induced urothelial cancer
Effect of FASN inhibition by orlistat on BCa cells: (a) orlistat exerts dose-dependent inhibition of clonogenic survival of the indicated BCa cell lines. Representative images of colonies > 50 cells after treatment with orlistat. Bar graphs report mean ± SEM of colony counts/well. All experiments were performed in triplicates/experimental condition and were repeated twice. (b) Line tracing of the oxygen consumption rate (OCR) of BCa cells treated with 20 µM orlistat or DMSO control. (c) Bar graphs report mean ± SEM of indicated measurements (n = 5/experimental condition) repeated twice. (d) Orlistat exerts dose-dependent inhibition of UMUC3 and T24T migration and matrix invasion. Bars report mean ± SEM of number of migrating and matrix-invading cells/high power field (HPF). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0–0001 and ns, not significant. One-way ANOVA with multiple comparisons (a) and Student’s t-test (c,d).
