Figure 1 | Scientific Reports

Figure 1

From: Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS

Figure 1

Experimental design (a), sphingolipid extraction workflow (b), and changes in body weight and survival rate (c), after virus inoculation. Mice were intranasally inoculated with PBS control or PBS comprising PR8 virus (50 or 500 PFU), and body weight was daily monitored until 6 dpi. A total of 15 mice/group were used for the experiment and 5 mice each were euthanized for sample collection on 1, 3, and 6 dpi. The mean body weight of mice is represented as a percentage of the original weight (c). White, gray, and black symbols indicate PBS control, 50 PFU, and 500 PFU PR8 virus infected mice, respectively; ****p < 0.0001, Mixed-effects analysis with Tukey’s multiple comparison test. (c) Percent survival of each group is shown. Black, orange, and red bars indicate PBS control, 50 PFU, and 500 PFU PR8 virus infected mice, respectively. Survival curves are compared using the log-rank (Mantel–Cox) analysis; *p < 0.05. Images are adopted from licensed BioRender.com.

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