Figure 2 | Scientific Reports

Figure 2

From: Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS

Figure 2

Concentrations of sphingolipids, gene expressions of S1P synthesis (Sphk1), and hydrolysis enzymes (Sgpl1) Concentrations of S1P and its precursor sphingosine in plasma (a), liver (b), and lung (c) of PR8 virus infected mice (n = 5 each, mean ± SE, Two-way ANOVA with Tukey’s multiple comparison test). Mice were intranasally inoculated with PBS alone or PBS comprising PR8 virus (500 PFU), and liver, e-WAT, lung, heart, and aorta samples were collected at 1, 3, and 6 dpi. Expressions of target genes (d) normalized with that of 18S are presented as fold changes relative to those of the control mice at each time point. Bars represent means ± SEM of 7 or 8 (liver, lung, aorta) or 3 or 4 (e-WAT, heart) animals. White and black bars indicate data from control and PR8 virus-infected mice, respectively; *p < 0.05, **p < 0.005, ***p < 0.0005, 2way-ANOVA on ddCt, control vs. PR8 virus-infected mice at each time point.

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