Figure 2
Blood glucose levels of mice with mutations of the insulin promoter and luciferase assay results. (A) Blood glucose levels of fed wild-type (n = 6) and heterozygous mice that had deletions in the Ins1/2 promoters (n = 6 each) at 12 weeks of age. (B) Blood glucose levels of fed wild-type (n = 6) and homozygous mice that had deletions in the Ins1/2 promoters (n = 6 each) at 12 weeks of age. (C) Mouse Ins1 promoter-luciferase plasmid containing approximately 1000 bp of the 5′-flanking sequences of the wild-type or mutated Ins1 promoter region was transfected into βTC6 cells (β-cell line). Forty-eight hours after transfection, cells were harvested and assayed (n = 3). **p < 0.01. (D) Mouse Ins2 promoter-containing luciferase plasmid containing approximately 1000 bp of the 5′-flanking sequences of the wild-type or Ins2 deletion promoter region was transfected into βTC6 cells (β-cell line). Forty-eight hours after transfection, cells were harvested and assayed (n = 3). **p < 0.01. The error bars represent the standard error.
