Figure 5 | Scientific Reports

Figure 5

From: In vivo evaluation of GG2–GG1/A2 element activity in the insulin promoter region using the CRISPR–Cas9 system

Figure 5

Ins1/2, Pdx1, Nkx2.2, and MafA mRNA levels in pancreatic islets from 1GG2GG mice. (A, B) qRT-PCR analysis of Ins1 and Ins2 in pancreatic islets of 1GG2GG mice [(A): male, (B): female]. (C, D) qRT-PCR analysis of Pdx1 expression in pancreatic islets of 1GG2GG mice [(C): male, (D): female]. (E, F) qRT-PCR analysis of Nkx2.2 expression in pancreatic islets of 1GG2GG mice [(E): male, (F): female]. (G, H) qRT-PCR analysis of MafA expression in pancreatic islets of 1GG2GG mice [(G): male, (H): female]. Pancreatic islets (purity > 95%) of wild-type mice served as a control. The data are expressed as the gene-to-Gapdh ratio; that of the control cells was arbitrarily defined as 1 (n = 8). The error bars represent the standard error. *p < 0.05.

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