Figure 1
Multilayered induction of TLR and IFN signaling pathways in response to C. albicans. (a), UMAP visualization of scRNAseq profiles colored according to cell cluster. (b) and (c), UMAP of resting and C. albicans-activated cell groups. DEGs, differentially expressed genes; IFN, interferon; ORA, overrepresentation analysis; scRNAseq, single-cell RNA sequencing; TLR, Toll-like receptor; UMAP, uniform manifold approximation and projection. (d), Dot plot showing pathways associated with the immune response to C. albicans, as obtained by ORA of DEGs. (e), Coexpression modules significantly enriched (M1-M11, and M13) in PBMCs (resting n = 30; C. albicans infected n = 24; dataset GSE42606). (f) and (g) Network representation of M1 and M2 with hubs (most connected genes) colored based on coexpression (blue color), coexpression and interactions (green color), or interactions only (dark-red color). (h) and (i), Enrichment representation obtained by modular genes coexpression in M1 and M2 showing significantly (− Log10 transformed adjusted p value) enriched signaling pathways. IFN, interferon; TLR, Toll-like receptor.
