Table 2 The p-values resulting from analysis of variance between three disintegration methods in each bacteria strain (BC—B. cereus; CG—C. glutamicum; EF—E. feacalis; EC—E. coli; KP—K. pneumoniae; PA—P. aeruginosa).
From: Comparison of bacteria disintegration methods and their influence on data analysis in metabolomics
Metabolite | G(+) | G(−) | ||||
|---|---|---|---|---|---|---|
BC | CG | EF | EC | KP | PA | |
5-Aminopentanoate | – | – | – | – | – | 2.90E−02 |
4-Aminobutyrate | 7.57E−01 | 1.21E−01# | 4.94E−03 | 7.33E−01# | 5.39E−02# | – |
Acetate | 1.90E−01 | 2.72E−01 | 8.56E−04 | 8.65E−03# | 3.52E−04 | 7.30E−05 |
Adenine | 2.91E−01# | 8.42E−03 | – | – | 2.05E−01 | – |
Adenosine | 8.31E−02 | – | 9.02E−03 | 1.93E−03# | 1.14E−01# | – |
Alanine | 3.43E−03 | 7.99E−01 | 1.28E−03 | 3.88E−02# | 4.24E−02# | 5.62E−04 |
AMP | – | 9.23E−02 | 5.47E−02# | 8.08E−03# | – | 3.57E−01 |
Asparagine | – | – | 1.17E−01 | – | – | – |
Aspartate | 5.70E−01 | 6.31E−01 | 1.05E−01 | – | – | 3.72E−01 |
Betaine | 7.62E−01 | 5.19E−01 | 6.82E−01 | – | 8.88E−01 | 8.05E−02# |
Cholate | 5.09E−02 | 2.99E−03# | 1.45E−01# | 1.93E−02# | 2.53E−02 | 3.65E−02# |
Formate | 3.48E−02 | 1.17E−07 | 1.98E−01 | 4.00E−02# | 9.85E−01# | 1.91E−03# |
Fumarate | – | – | – | – | 8.06E−01 | – |
Glutamate | 7.56E−01 | 4.48E−01 | 2.33E−01 | – | – | 2.72E−01 |
Glutamine | – | – | 7.09E−01 | – | – | – |
Glycine | 1.04E−01# | 6.78E−01 | 1.37E−01# | 9.44E−03# | 2.31E−02 | 4.02E−01 |
Histidine | 9.21E−01 | 5.25E−03# | 1.40E−01# | 4.03E−01# | 6.14E−02 | – |
Inosine | 5.21E−02 | 3.54E−02# | 1.24E−02 | – | – | – |
Isocitrate | – | – | – | – | – | 1.06E−02# |
Isoleucine | 1.90E−01 | 6.26E−01 | 1.78E−01 | 9.97E−02# | 1.18E−01 | 8.24E−01 |
Lactate | 3.55E−01 | 9.35E−03 | 5.41E−01 | 1.83E−02# | 7.66E−01 | 2.68E−01 |
Leucine | 5.48E−01 | 1.54E−01 | 1.82E−01# | 1.25E−01# | 2.46E−01 | 6.43E−02 |
Lysine | 6.97E−01 | 9.14E−01# | 4.93E−03 | 1.28E−02# | 3.80E−02# | 4.49E−06 |
Methionine | 5.90E−05 | 9.63E−03# | 3.07E−03# | 1.07E−15# | 3.68E−03 | 5.92E−04 |
NAD+ | 9.97E−02# | 1.79E−02 | 2.30E−01# | 7.11E−03# | 3.66E−02 | 4.62E−01 |
Nicotinate | – | – | – | – | 2.30E−01# | – |
O-Phosphocholine | 4.58E−02 | 2.06E−01 | 5.93E−01 | 2.00E−02# | 3.20E−01# | 5.36E−03 |
Oxypurinol | 5.48E−02 | 8.25E−03 | – | – | 4.06E−01 | – |
Phenylalanine | 3.00E−01 | 9.22E−01 | 8.05E−01 | 9.14E−01# | 4.24E−01 | 2.95E−01 |
Propyleneglycol | 7.81E−03 | – | – | – | 1.37E−01 | – |
Pyruvate | 4.12E−01 | 5.08E−01 | 4.53E−02# | 3.07E−03# | 3.25E−01# | 5.78E−01 |
Sarcosine | 4.47E−03# | 9.91E−01 | 1.10E−02 | – | 1.12E−01 | 1.53E−01 |
Succinate | 6.80E−01 | 4.16E−01 | 4.39E−01 | 6.13E−02# | 3.68E−01# | 2.87E−01 |
Threonine | 9.18E−01 | 4.39E−01 | 9.05E−01 | 1.68E−01# | 6.77E−01# | 1.38E−01 |
Trehalose | – | 5.10E−01# | – | – | – | – |
Tyramine | 9.05E−01 | 7.50E−01 | 5.65E−01 | – | 2.93E−01 | 3.53E−01 |
Tyrosine | 4.00E−01 | 9.22E−01 | 4.62E−01 | – | 3.67E−01 | 5.59E−01 |
UDP-glucose | 7.57E−01 | 4.63E−01 | – | 6.99E−03# | – | – |
Uracil | 5.59E−01 | – | – | 2.31E−01# | 9.64E−01 | 1.45E−01 |
Uridine | 1.00E−02 | – | – | – | – | – |
Valine | 1.88E−01 | 4.48E−01 | 2.72E−01 | 5.39E−02# | 5.31E−02# | 2.24E−01 |
β-Alanine | 8.99E−01 | 8.99E−01 | – | – | – | – |
