Figure 5
Reduction in aquaporin expression and cell damage by HMGB1. (a) Cell viability of chondrogenic C3H10T1/2 cells by HMGB1. (b) Expressions of AQP1, -3 and -4 mRNA by HMGB1. (c) Western blot analyses for AQP1, -3 and -4 protein expression by HMGB1. Densitometric analyses of the western blots are shown on the right. (d) HMGB1 and AQP4 protein expression in young and old aged cartilage tissues (n = 5 per group). Densitometric analyses of the western blots are shown on the right. (e) Morphology of chondrogenic C3H10T1/2 cells by HMGB1 administration. (f) Flow cytometry analysis in HMGB1-treated C3H10T1/2 cells. Forward scatter (FSC) represent the cell size, and side scatter (SSC) data represent the complexity or granularity of the cell.
