Figure 4
From: Dried blood sample analysis by antibody array across the total testing process
Optimization of Elution buffer composition for protein extraction in DBS samples. 4 different elution buffers: 1 × PBS (EB1), 1 × PBS containing 0.1% Tween-20 (EB2), 1 × PBS containing 10% casein and 50% BSA (EB3), and 1 × PBS containing 10% casein, 50% BSA, and 0.1% Tween-20 (EB4) were used to elute 3 DBS samples each. (A) The number and percentage of 40 proteins that were quantitatively assessed and measurable with each elution buffer. (B) Concentrations and variance as evidenced by the standard error of 5 proteins compared across the 4 different elution buffers.
