Figure 4 | Scientific Reports

Figure 4

From: Quantifying protein abundance on single cells using split-pool sequencing on DNA-barcoded antibodies for diagnostic applications

Figure 4

QBC2 CLR-transformed antibody scores and flow cytometry from patient 1 bone marrow sample. (A) Cells from patient 1 are gated for CD45+ expression. Further stratification of B-cells in this population shows that CD19+ CD3− gated B-cells show B-cells that have moderate CD8 expression (red) (isotype control expression on CD20+ cells in blue for comparison). (B) CD20+ gated B-cells show a pathological expansion of the kappa light chain population. (C) Flow cytometry data for the same sample. Monocytes, granulocytes and lymphocytes are identified; lymphocytes are gated for downstream analysis. Population proportion of CD19+ CD3− as identified in flow cytometry similar to QBC2 (85% and 84%, respectively, from panel A). (D) Bright CD20 expression gated to capture B-cell population. Flow cytometry demonstrates a kappa restricted B cell population, concordant with clonal expansion identified in QBC2.

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