Figure 5 | Scientific Reports

Figure 5

From: Quantifying protein abundance on single cells using split-pool sequencing on DNA-barcoded antibodies for diagnostic applications

Figure 5

QBC2 CLR-transformed antibody scores and flow cytometry from patient 9 bone marrow sample. (A) Cells from patient 9 are gated for CD45+ expression. Gating for T-cells using CD3+ CD19− reveals an expanded CD4+ T-cell population. (B) Large proportion of cells revealed CD4+ CD25+ phenotype. (C) Population of CD3+ T-cells showed elevated PD-1 and variable CTLA-4 (red) (isotype control in blue for comparison). (D) Flow cytometry of the same sample. Cells were gated for lymphocytes, CD45+. Comparison between QBC2 and flow cytometry showed high concordance in gated populations. Population proportions between QBC2 and flow cytometry are consistent throughout.

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