Figure 1 | Scientific Reports

Figure 1

From: Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal–epidermal junction

Figure 1

Age-dependent change of collagen fibrils in dermis. Immunostaining of type I procollagen (A–C), type III collagen (D–F) and type V collagen (G–I) in sun-protected young skin (A, D, G), sun-protected older skin (B, E, H) and sun-exposed older skin (C, F, I). Bars: 100 μm. Staining signal intensities of type I procollagen (J), type III collagen (K) and type V collagen (L) were analyzed using WINROOF 2015 image-analyzing software (Mitani, Fukui, Japan, https://www.mitani-visual.jp/products/image_analys_ismeasure-ment/winroof/). Data are the means ± SD of six images from six donors each in the 20–30’s and 60–70’s. * p < 0.05, ** p < 0.01. mRNA expression levels of COL1A1 (M), COL3A1 (N) and COL5A1 (O) genes in cultured human fibroblasts from young and older donors were analyzed by qPCR. Data are the means ± SD of three independent experiments from six donors. *p < 0.05, **p < 0.01.

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