Figure 5 | Scientific Reports

Figure 5

From: Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal–epidermal junction

Figure 5

Promotion of collagen expression in fibroblasts by conditioned medium from the skin equivalent model. mRNA expression levels of the COL1A1 (A), COL3A1 (B) and COL5A1 (C) genes in cultured fibroblasts cultured with conditioned media from the skin equivalent model were analyzed by qPCR. Data are expressed as mean ± SD of three independent experiments. * p < 0.05. Protein membrane array analysis was performed by using the cell lysate from epidermis of the skin equivalent model cultured with MMP inhibitor CGS27023A and heparinase inhibitor BIPBIPU (D). mRNA expression levels of the COL1A1 (E), COL3A1 (F) and COL5A1 (G) genes in cytokine-stimulated fibroblasts were analyzed by qPCR. Data are expressed as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.

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