Figure 2 | Scientific Reports

Figure 2

From: Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay

Figure 2

The optimum incubation periods, detection limits and FAW species specificities for the Sf4p LAMP assay. (a) After 35 min incubation, the fluorescence signals and the patterns of ladder-shaped DNA fragments were similar among Sf4p LAMP assays. No amplification was detected after 50 min of incubation when genomic DNA was not added (Control = Con). (b) When serially diluted FAW genomic DNAs were used to determine detection limits for the Sf40 LAMP assay, fluorescence signals and DNA amplification were detected above 0.1 ng/µl of FWA genomic DNA. The fluorescence intensities and patterns of DNA fragments were similar above 1 ng/µl of FAW genomic DNA. (c) When genomic DNAs of moths captured by FAW sex-pheromone traps (1–10) or raised in the insectarium (11–15) were used for the Sf4p LAMP assay, strong fluorescence signals and amplified DNA fragments were observed from FAW genomic DNA. Moth species used in this study were as follows: 1. FAW, 2. Axylia putris, 3. Mythimna loreyi, 4. Discestra trifolii, 5. Hermonassa cecilia, 6. Anomis flava, 7. Aedia leucomelas, 8. Pleuroptya ruralis, 9. Mythimna separata, 10. Lacanobia contigua, 11. Helicoverpa armigera, 12. Mamestra brassicae, 13. Spodoptera exigua, 14. Spodoptera litura, 15. Plutella xylostella. The red arrows indicate the contamination-free SYBR green delivery device.

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