Figure 3

Variability of the three Spinacia species based on the chloroplast genome. (a) The purple lines in the innermost circle correspond to variants between S. turkestanica and S. oleracea. The green region in the outermost circle corresponds to variants between S. tetrandra and S. oleracea with a 1-Kb window and 100-bp steps. Genes represented by blue rectangles are on the positive strand while genes represented by red rectangles are on the negative strand. (b) Genetic distance between S. oleracea and the wild relative chloroplast assemblies of S. tetrandra (red) and S. turkestanica (blue). (c) The phylogenetic tree was constructed based on three variants between S. turkestanica and S. oleracea using 85 Spinacia accessions, including eight S. tetrandra, 16 S. turkestanica, and 61 S. oleracea individuals. Yellow rectangles indicate that the homozygous genotype in the accession was consistent with the reference chloroplast genome, whereas blue rectangles indicate that the homozygous genotype differed from the reference chloroplast genome.