Figure 4 | Scientific Reports

Figure 4

From: Long noncoding RNA ADAMTS9-AS1 represses ferroptosis of endometrial stromal cells by regulating the miR-6516-5p/GPX4 axis in endometriosis

Figure 4

ADAMTS9-AS1 repressed ferroptosis of ESCs by regulating GPX4. The Fe2+ levels (A), MDA content (B), and ROS levels (C) were assayed using the indicated kits for NESCs and EESCs. The ROS levels (D) and MDA content (E) were assessed using the indicated kits for EESCs after ADAMTS9-AS1 knockdown. (F) The mRNA levels of COX-2, ACSL4, PTGS2, NOX1, GPX4, and FTH1 were assayed using qPCR analysis in EESCs after ADAMTS9-AS1 knockdown. (G,H) The protein level of GPX4 was assayed using western blot analysis in EESCs after ADAMTS9-AS1 knockdown. *p < 0.05, **p < 0.01.

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