Table 1 Differences of amino acids concentrations (pM) in vitro (n = 5) and in vivo (n = 5) between normal and cancer conditions across 24 hours.
From: Presence of human breast cancer xenograft changes the diurnal profile of amino acids in mice
Amino acids | In vitro | In vivo | ||||||
|---|---|---|---|---|---|---|---|---|
Time of sampling (CT) | MCF10A | MDA-MB-231 | p value | Time of day (ZT) | Non-tumor bearing mice | Tumor bearing mice | p value | |
Alanine (Ala) HMDB0000161 | 4 | 161.4 ± 32.0 | 26.6 ± 3.6 | p < 0.001 | 0 | 646.2 ± 51.2 | 859.0 ± 114.7 | p < 0.05 |
6 | 180.6 ± 31.6 | 29.2 ± 3.6 | p < 0.001 | 3 | 561.6 ± 133.5 | 809.7 ± 102.6 | p < 0.05 | |
16 | 208.8 ± 28.0 | 34.3 ± 5.3 | p < 0.001 | 6 | 853.0 ± 73.1 | 624.0 ± 96.6 | p < 0.01 | |
24 | 248.7 ± 15.5 | 39.4 ± 4.5 | p < 0.001 | 9 | 435.8 ±118.7 | 299.6 ± 73.4 | N/S | |
- | – | – | – | 12 | 633.6 ± 56.7 | 495.3 ± 94.3 | N/S | |
– | – | – | – | 15 | 522.0 ± 34.2 | 563.6 ± 67.5 | N/S | |
– | – | – | – | 18 | 820.6 ± 58.3 | 704.3 ± 123.0 | N/S | |
– | – | – | – | 21 | 774.4 ± 167.2 | 709.6 ± 114.1 | N/S | |
Arginine (Arg) HMDB0000517 | 4 | 51.8 ± 11.4 | 28.7 ± 8.7 | p < 0.05 | 0 | 194.7 ± 14.6 | 267.8 ± 33.9 | p < 0.01 |
6 | 58.8 ± 14.2 | 25.6 ± 10.6 | p < 0.001 | 3 | 141.0 ± 36.0 | 287.0 ± 35.1 | p < 0.001 | |
16 | 54.2 ± 14.2 | 25.4 ± 4.8 | p < 0.01 | 6 | 208.3 ± 2.1 | 135.7 ± 32.1 | p < 0.01 | |
24 | 43.6 ± 1.6 | 25.6 ± 7.6 | N/S | 9 | 100.6 ± 30.3 | 49.4 ± 9.4 | N/S | |
– | – | – | – | 12 | 147.5 ± 7.1 | 103.2 ± 36.4 | N/S | |
– | – | – | – | 15 | 97.5 ± 20.6 | 100.5 ± 17.8 | N/S | |
– | – | – | – | 18 | 269.7 ± 23.0 | 203.7 ± 35.1 | p < 0.05 | |
– | – | – | – | 21 | 247.7 ± 10.9 | 219.8 ± 35.4 | N/S | |
Asparagine (Asn) HMDB0000168 | 4 | 8.5 ± 1.8 | 12.2 ± 1.2 | p < 0.01 | 0 | 37.9 ± 5.2 | 49.6 ± 7.7 | N/S |
6 | 9.6 ± 2.1 | 12.13 ± 1.7 | N/S | 3 | 41.5 ± 12.9 | 61.6 ± 6.3 | p < 0.001 | |
16 | 8.1 ± 1.6 | 12.0 ± 1.3 | p < 0.01 | 6 | 54.9 ± 4.8 | 39.0 ± 5.1 | p < 0.01 | |
24 | 6.7 ± 0.5 | 12.5 ± 1.5 | p < 0.001 | 9 | 28.3 ± 4.9 | 25.5 ± 5.9 | N/S | |
– | – | – | – | 12 | 46.6 ± 5.5 | 25.2 ± 7.5 | p < 0.001 | |
– | – | – | – | 15 | 31.9 ± 4.4 | 34.8 ± 4.7 | N/S | |
– | – | – | – | 18 | 47.9 ± 2.6 | 34.6 ± 3.3 | p < 0.05 | |
– | – | – | – | 21 | 63.5 ± 7.8 | 52.5 ± 7.7 | N/S | |
Aspartate (Asp) HMDB0000191 | 4 | 19.3 ± 2.2 | 15.1 ± 3.0 | N/S | 0 | 7.1 ± 1.3 | 10.6 ± 2.8 | N/S |
6 | 19.7 ± 5.1 | 21.9 ± 3.0 | N/S | 3 | 3.8 ± 1.5 | 10.0 ± 1.9 | p < 0.01 | |
16 | 10.2 ± 4.1 | 17.3 ± 3.1 | p < 0.05 | 6 | 7.5 ± 2.2 | 12.5 ± 12.6 | p < 0.01 | |
24 | 6.2 ± 2.7 | 19.2 ± 5.0 | p < 0.001 | 9 | 3.7 ± 1.2 | 5.5 ± 0.2 | N/S | |
– | – | – | – | 12 | 6.9 ± 2.7 | 5.5 ± 1.6 | N/S | |
– | – | – | – | 15 | 4.9 ± 1.6 | 4.4 ± 2.5 | N/S | |
– | – | – | – | 18 | 8.3 ± 2.8 | 7.2 ± 2.0 | N/S | |
– | – | – | – | 21 | 7.3 ± 1.7 | 3.7 ± 1.2 | N/S | |
Citrulline (Cit) HMDB0000904 | 4 | – | – | – | 0 | 78.6 ± 8.7 | 117.8 ± 14.8 | p < 0.001 |
6 | – | – | – | 3 | 73.4 ± 16.9 | 106.7 ± 18.5 | p < 0.01 | |
16 | – | – | 6 | 94.3 ± 5.9 | 66.4 ± 14.8 | p < 0.01 | ||
24 | – | – | – | 9 | 57.6 ± 7.2 | 43.2 ± 10.5 | N/S | |
– | – | – | – | 12 | 76.5 ± 15.0 | 54.4 ± 12.0 | p < 0.05 | |
– | – | – | – | 15 | 56.0 ± 7.4 | 68.9 ± 8.5 | N/S | |
– | – | – | – | 18 | 115.7 ± 13.3 | 88.8 ± 0.8 | N/S | |
– | – | – | – | 21 | 110.2 ± 9.1 | 94.2 ± 12.4 | N/S | |
Glutamine (Gln) HMDB0000641 | 4 | 101.4 ± 32.1 | 473.5 ± 68.0 | p < 0.001 | 0 | 876.3 ± 82.0 | 1029.7 ± 153.7 | N/S |
6 | 90.8 ± 47.9 | 483.8 ± 111.1 | p < 0.001 | 3 | 907.6 ± 89.5 | 998.8 ± 90.3 | N/S | |
16 | 99.2 ± 31.6 | 448.5 ± 47.8 | p < 0.001 | 6 | 926.3 ± 53.9 | 788.5 ± 76.3 | N/S | |
24 | 112.6 ± 13.4 | 466.4 ± 86.0 | p < 0.001 | 9 | 708.0 ± 50.4 | 787.7 ± 143.3 | N/S | |
– | – | – | – | 12 | 808.6 ± 24.7 | 846.2 ± 92.3 | N/S | |
– | – | – | – | 15 | 861.2 ± 28.0 | 916.6 ± 55.2 | N/S | |
– | – | – | – | 18 | 1069.0 ± 76.9 | 1133.0 ± 163.5 | N/S | |
– | – | – | – | 21 | 1071.2 ± 157.2 | 818.3 ± 140.3 | p < 0.05 | |
Glutamate (Glu) HMDB0000148 | 4 | 124.8 ± 20.1 | 166.0 ± 39.5 | N/S | 0 | 30.8 ± 4.0 | 33.0 ± 9.2 | N/S |
6 | 141.3 ± 27.1 | 213.9 ± 36.8 | p < 0.01 | 3 | 27.0 ± 5.8 | 24.3 ± 3.7 | N/S | |
16 | 126.4 ± 44.2 | 206.7 ± 19.9 | p < 0.01 | 6 | 22.8 ± 7.1 | 44.6 ± 4.7 | p < 0.001 | |
24 | 167.6 ± 27.0 | 221.5 ± 8.1 | N/S | 9 | 24.7 ± 6.6 | 42.5 ± 13.7 | p < 0.01 | |
– | – | – | – | 12 | 31.5 ± 2.5 | 22.2 ± 4.7 | N/S | |
– | – | – | – | 15 | 26.2 ± 5.1 | 23.3 ± 7.3 | N/S | |
– | – | – | – | 18 | 27.4 ± 2.8 | 44.0 ± 9.6 | p < 0.05 | |
– | – | – | – | 21 | 34.2 ± 7.6 | 36.7 ± 6.1 | N/S | |
Glycine (Gly) HMDB0000123 | 4 | 85.6 ± 8.3 | 118.6 ± 2.3 | p < 0.001 | 0 | 333.4 ± 21.2 | 319.2 ± 50.1 | N/S |
6 | 100.6 ± 47.9 | 135.1 ± 13.8 | p < 0.001 | 3 | 283.0 ± 30.0 | 347.4 ± 24.2 | N/S | |
16 | 84.8 ± 31.6 | 150.6 ± 15.6 | p < 0.001 | 6 | 386.2 ± 26.1 | 305.0 ± 53.3 | p < 0.05 | |
24 | 75.2 ± 13.4 | 167.6 ± 10.9 | p < 0.001 | 9 | 266.6 ± 26.4 | 237.0 ± 48.2 | N/S | |
– | – | – | – | 12 | 285.8 ± 41.0 | 234.0 ± 49. | N/S | |
– | – | – | – | 15 | 217.2 ± 10.1 | 263.0 ± 19.0 | N/S | |
– | – | – | – | 18 | 299.0 ± 38.9 | 251.2 ± 52.3 | N/S | |
– | – | – | – | 21 | 319.8 ± 73.8 | 262.2 ± 48.8 | N/S | |
Histidine (His) HMDB0000177 | 4 | 18.9 ± 1.9 | 21.5 ± 4.1 | N/S | 0 | 89.3 ± 11.8 | 97.7 ± 17.1 | N/S |
6 | 22.0 ± 2.7 | 21.2 ± 5.3 | N/S | 3 | 86.3 ± 9.4 | 105.3 ± 15.8 | N/S | |
16 | 20.7 ± 2.0 | 22.2 ± 3.2 | N/S | 6 | 91.2 ± 7.2 | 78.5 ± 13.9 | N/S | |
24 | 19.9 ± 1.5 | 22.8 ± 4.1 | N/S | 9 | 65.6 ± 10.3 | 68.0 ± 12.5 | N/S | |
– | – | – | – | 12 | 84.6 ± 7.3 | 77.0 ± 11.8 | N/S | |
– | – | – | – | 15 | 80.1 ± 2.7 | 90.1 ± 8.5 | N/S | |
– | – | – | – | 18 | 108.3 ± 8.8 | 97.3 ± 20.6 | N/S | |
– | – | – | – | 21 | 111.4 ± 20.6 | 100.0 ± 14.7 | N/S | |
Isoleucine (Ile) HMDB0000172 | 4 | 45.4 ± 5.3 | 85.3 ± 17.1 | p<0.01 | 0 | 135.0 ± 17.4 | 183.0 ± 6.1 | P<0.01 |
6 | 54.1 ± 8.7 | 83.4 ± 24.4 | P < 0.05 | 3 | 111.0 ± 32.2 | 174.3 ± 19.4 | P<0.001 | |
16 | 46.3 ± 5.1 | 87.8 ± 13.1 | P<0.001 | 6 | 127.7 ± 5.5 | 86.2 ± 29.1 | N/S | |
24 | 42.6 ± 3.3 | 87.6 ± 16.7 | P<0.001 | 9 | 105.6 ± 15.5 | 104.4 ± 10.0 | N/S | |
– | – | – | – | 12 | 177.7 ± 48.0 | 115.9 ± 26.1 | p<0.001 | |
– | – | – | – | 15 | 109.0 ± 11.4 | 114.3 ± 14.4 | N/S | |
– | – | – | – | 18 | 178.2 ± 10.1 | 135.0 ± 11.8 | P < 0.05 | |
– | – | – | – | 21 | 218.3 ± 6.5 | 170.2 ± 25.7 | p<0.01 | |
Leucine (Leu) HMDB0000687 | 4 | 63.6 ± 17.6 | 101.8 ± 17.0 | P < 0.05 | 0 | 200.6 ± 17.7 | 297.7 ± 17.2 | p<0.01 |
6 | 72.2 ± 19.2 | 99.4 ± 30.7 | N/S | 3 | 147.5 ± 46.0 | 261.7 ± 28.3 | p<0.001 | |
16 | 61.6 ± 20.4 | 108.2 ± 18.8 | P < 0.05 | 6 | 208.0 ± 4.0 | 137.1 ± 53.1 | N/S | |
24 | 48.2 ± 12.1 | 103.1 ± 21.1 | p<0.01 | 9 | 146.2 ± 26.9 | 147.0 ± 10.8 | N/S | |
– | – | – | – | 12 | 250.2 ± 59.0 | 153.7 ± 29.2 | p<0.01 | |
– | – | – | – | 15 | 153.0 ± 15.6 | 168.8 ± 9.9 | N/S | |
– | – | – | – | 18 | 290.5 ± 18.6 | 213.7 ± 12.9 | P < 0.05 | |
– | – | – | – | 21 | 276.4 ± 63.9 | 257.0 ± 36.5 | N/S | |
Lysine (Lys) HMDB0000182 | 4 | 45.6 ± 12.4 | 83.7 ± 27.3 | N/S | 0 | 506.2 ± 22.5 | 694.0 ± 71.7 | p<0.01 |
6 | 53.4 ± 15.8 | 73.0 ± 34.5 | N/S | 3 | 470.0 ± 69.0 | 709.0 ± 69.8 | p<0.001 | |
16 | 49.9 ± 15.7 | 75.6 ± 16.5 | N/S | 6 | 610.6 ± 34.5 | 384.6 ± 92. | p<0.001 | |
24 | 33.6 ± 9.8 | 78.1 ± 27.0 | p<0.05 | 9 | 266.4 ± 63.9 | 224.6 ± 42.9 | N/S | |
– | – | – | – | 12 | 369.0 ± 11.5 | 258.7 ± 90.0 | N/S | |
– | – | – | – | 15 | 269.6 ± 26.6 | 305.8 ± 43.2 | N/S | |
– | – | – | – | 18 | 520.4 ± 61.3 | 368.0 ± 90.4 | p<0.01 | |
– | – | – | – | 21 | 553.0 ± 85.9 | 492.0 ± 42.2 | N/S | |
Methionine (Met) HMDB0000696 | 4 | 8.3 ± 2.2 | 13.3 ± 2.1 | N/S | 0 | 72.7 ± 8.9 | 106.3 ± 6.6 | P < 0.05 |
6 | 7.7 ± 2.0 | 11.4 ± 4.2 | N/S | 3 | 69.2 ± 20.0 | 115.7 ± 21.2 | p<0.001 | |
16 | 9.0 ± 2.5 | 11.8 ± 1.2 | N/S | 6 | 79.1 ± 6.9 | 47.5 ± 8.0 | P < 0.05 | |
24 | 7.6 ± 1.7 | 10.1 ± 3.2 | N/S | 9 | 38.1 ± 5.1 | 27.0 ± 5.9 | N/S | |
– | – | – | – | 12 | 54.4 ± 5.7 | 38.0 ± 5.4 | N/S | |
– | – | – | – | 15 | 51.4 ± 12.4 | 48.2 ± 6.7 | N/S | |
– | – | – | – | 18 | 120.7 ± 7.5 | 99.3 ± 11.1 | N/S | |
– | – | – | – | 21 | 102.6 ± 16.7 | 85.4 ± 32.9 | N/S | |
Ornithine (Orn) HMDB0000214 | 4 | – | – | – | 0 | 101.5 ± 17.5 | 159.0 ± 17.6 | p<0.01 |
6 | – | – | – | 3 | 96.4 ± 26.5 | 171.0 ± 23.6 | p<0.001 | |
16 | – | – | – | 6 | 91.6 ± 9.0 | 53.3 ± 17.0 | P < 0.05 | |
24 | – | – | – | 9 | 50.0 ± 11.2 | 37.4 ± 8.0 | N/S | |
– | – | – | – | 12 | 98.9 ± 15.4 | 52.3 ± 23.8 | P < 0.05 | |
– | – | – | – | 15 | 66.8 ± 15.8 | 78.8 ± 21.2 | N/S | |
– | – | – | – | 18 | 162.4 ± 20.3 | 90.7 ± 11.1 | p<0.001 | |
– | – | – | – | 21 | 158.6 ± 31.9 | 152.4 ± 23.9 | N/S | |
Phenylalanine (Phe) HMDB0000159 | 4 | 27.1 ± 2.8 | 45.9 ± 8.4 | p<0.01 | 0 | 143.0 ± 9.2 | 184.5 ± 25.6 | P < 0.05 |
6 | 33.4 ± 6.1 | 45.9 ± 11.9 | N/S | 3 | 126.0 ± 30.7 | 183.7 ± 7.1 | p<0.01 | |
16 | 30.0 ± 3.0 | 48.4 ± 7.5 | p<0.01 | 6 | 160.7 ± 1.5 | 114.8 ± 30.9 | P < 0.05 | |
24 | 29.1 ± 2.0 | 48.0 ± 9.1 | p<0.01 | 9 | 112.0 ± 4.5 | 95.0 ± 4.6 | N/S | |
– | – | – | – | 12 | 175.7 ± 17.0 | 133.7 ± 28.0 | P < 0.05 | |
– | – | – | – | 15 | 126.0 ± 10.2 | 132.0 ± 13.4 | N/S | |
– | – | – | – | 18 | 194.8 ± 11.7 | 154.0 ± 20.2 | P < 0.05 | |
– | – | – | – | 21 | 178.6 ± 20.6 | 174.0 ± 20.5 | N/S | |
Proline (Pro) HMDB0000162 | 4 | 28.1 ± 1.5 | 23.0 ± 1.3 | N/S | 0 | 146.0 ± 18.3 | 208.0 ± 30.4 | p<0.01 |
6 | 30.0 ± 1.7 | 28.9 ± 2.1 | N/S | 3 | 84.8 ± 28.5 | 135.5 ± 25.3 | P < 0.05 | |
16 | 25.8 ± 4.4 | 35.2 ± 4.1 | p<0.01 | 6 | 175.6 ± 11.1 | 117.1 ± 28.1 | p<0.001 | |
24 | 29.5 ± 1.2 | 42.3 ± 4.1 | p<0.001 | 9 | 62.0 ± 8.4 | 44.4 ± 12.2 | N/S | |
– | – | – | – | 12 | 161.0 ± 39.3 | 107.2 ± 4.0 | p<0.01 | |
– | – | – | – | 15 | 69.7 ± 10.9 ± | 80.2 ± 10.4 | N/S | |
– | – | – | – | 18 | 210.4 ± 19.5 | 150.3 ± 19.3 | p<0.01 | |
– | – | – | – | 21 | 150.0 ± 29.6 | 116.3 ± 19.1 | N/S | |
Serine (Ser) HMDB0000187 | 4 | 21.6 ± 1.4 | 64.4 ± 8.2 | p<0.001 | 0 | 170.3 ± 7.1 | 206.7 ± 22.5 | N/S |
6 | 28.7 ± 4.9 | 68.7 ± 14.7 | p<0.001 | 3 | 159.5 ± 22.5 | 215.4 ± 13.5 | p<0.01 | |
16 | 16.4 ± 2.0 | 60.7 ± 6.3 | p<0.001 | 6 | 232.8 ± 23.8 | 160.4 ± 35.5 | p<0.001 | |
24 | 7.9 ± 1.1 | 61.6 ± 9.4 | p<0.001 | 9 | 115.8 ± 18.1 | 93.3 ± 23.3 | N/S | |
– | – | – | – | 12 | 159.8 ± 9.4 | 101.7 ± 24.7 | p<0.01 | |
– | – | – | – | 15 | 126.8 ± 13.8 | 135.6 ± 14.7 | N/S | |
– | – | – | – | 18 | 201.3 ± 11.0 | 155.8 ± 25.8 | P < 0.05 | |
– | – | – | – | 21 | 220.5 ± 24.1 | 179.2 ± 32.1 | N/S | |
Threonine (Thr) HMDB0000167 | 4 | 61.1 ± 4.9 | 94.5 ± 11.5 | p<0.01 | 0 | 175.4 ± 21.3 | 190.8 ± 40.9 | N/S |
6 | 72.4 ± 7.8 | 101.7 ± 15.5 | p<0.01 | 3 | 176.6 ± 42.5 | 202.0 ± 36.9 | N/S | |
16 | 65.2 ± 4.7 | 101.5 ± 15.3 | p<0.001 | 6 | 173.4 ± 16.7 | 129.9 ± 26.9 | N/S | |
24 | 64.0 ± 1.8 | 108.4 ± 16.2 | p<0.001 | 9 | 114.5 ± 17.9 | 85.4 ± 20.4 | N/S | |
– | – | – | – | 12 | 138.8 ± 19.5 | 110.3 ± 24.2 | N/S | |
– | – | – | – | 15 | 138.8 ± 19.5 | 147.8 ± 18.2 | N/S | |
– | – | – | – | 18 | 226.0 ± 12.6 | 197.8 ± 62.5 | N/S | |
– | – | – | – | 21 | 238.0 ± 30.9 | 223.4 ± 22.1 | N/S | |
Tryptophan (Trp) HMDB0000929 | 4 | 6.6 ± 0.4 | 8.3 ± 1.0 | N/S | 0 | 116.8 ± 20.9 | 132.4 ± 15.9 | N/S |
6 | 7.8 ± 0.6 | 8.8 ± 1.6 | N/S | 3 | 111.5 ± 32.7 | 123.6 ± 25.7 | N/S | |
16 | 6.8 ± 1.1 | 9.0 ± 1.0 | P < 0.05 | 6 | 111.2 ± 20.1 | 101.5 ± 21.8 | N/S | |
24 | 7.1 ± 0.5 | 9.3 ± 1.2 | P < 0.05 | 9 | 98.4 ± 10.3 | 121.1 ± 34.9 | N/S | |
– | – | – | – | 12 | 130.8 ± 26.2 | 129.2 ± 30.9 | N/S | |
– | – | – | – | 15 | 76.6 ± 7.8 | 70.7 ± 7.2 | N/S | |
– | – | – | – | 18 | 132.0 ± 13.7 | 129.2 ± 36.6 | N/S | |
– | – | – | – | 21 | 126.2 ± 23.5 | 115.2 ± 19.4 | N/S | |
Tyrosine (Tyr) HMDB0000158 | 4 | 28.1 ± 2.9 | 42.8 ± 7.9 | p<0.01 | 0 | 143.0 ± 16.8 | 174.0 ± 46.7 | N/S |
6 | 33.1 ± 4.5 | 38.0 ± 5.7 | N/S | 3 | 131.9 ± 28.0 | 149.4 ± 15.2 | N/S | |
16 | 30.4 ± 3.1 | 45.3 ± 6.5 | p<0.01 | 6 | 139.8 ± 25.9 | 102.2 ± 17.3 | p<0.001 | |
24 | 30.3 ± – 2.0 | 43.0 ± 2.0 | p<0.01 | 9 | 135.5 ± 45.1 | 65.7 ± 14.9 | N/S | |
– | – | – | – | 12 | 175.4 ± 18.7 | 86.9 ± 30.7 | N/S | |
– | – | – | – | 15 | 78.6 ± 11.6 | 72.0 ± 7.9 | N/S | |
– | – | – | – | 18 | 96.2 ± 22.9 | 131.4 ± 45.6 | N/S | |
– | – | – | – | 21 | 72.0 ± 10.0 | 117.1 ± 17.7 | N/S | |
Valine (Val) HMDB0000883 | 4 | 26.3 ± 2.7 | 93.1 ± 17.6 | p<0.001 | 0 | 263.3 ± 32.7 | 313.7 ± 18.0 | N/S |
6 | 32.0 ± 4.9 | 91.0 ± 22.9 | p<0.001 | 3 | 217.0 ± 64.5 | 343.3 ± 23.4 | P < 0.05 | |
16 | 27.9 ± 2.2 | 96.8 ± 14.7 | p<0.001 | 6 | 267.7 ± 16.3 | 189.7 ± 56.3 | N/S | |
24 | 23.6 ± 2.2 | 96.7 ± 16.2 | p<0.001 | 9 | 228.2 ± 27.0 | 213.8 ± 23.0 | p<0.001 | |
– | – | – | – | 12 | 311.0 ± 37.9 | 251.8 ± 43.0 | P < 0.05 | |
– | – | – | – | 15 | 267.0 ± 13.3 | 233.3 ± 17.6 | N/S | |
– | – | – | – | 18 | 354.4 ± 16.8 | 285.7 ± 28.5 | N/S | |
– | – | – | – | 21 | 433.0 ± 13.1 | 329.5 ± 71.4 | N/S | |
