Figure 2 | Scientific Reports

Figure 2

From: Dielectricity of a molecularly crowded solution accelerates NTP misincorporation during RNA-dependent RNA polymerization by T7 RNA polymerase

Figure 2

Polymerization in the absence of PEG200 by T7 RNAP with (a) primer A, (b) primer C, (c) primer G, (d) primer U, and in the presence of PEG200 with (e) primer A, (f) primer C, (g) primer G, (h) primer U. Positions of products (n + 1, 2, 3, or 4, where n is the primer length) are indicated. Conditions: 0.5 μM T7 RNAP, 0.5 μM each RNA primer, 0.5 μM template RNA with 100 μM each NTP in 50 mM Tris–HCl (pH 8.0), 10 mM MgCl2, and with or without 20wt% PEG200 at 25 °C for 12 h. The results using primer G are the original data of PAGE, which was not shown in the previous report26. Full-length gels are presented in Supplementary Information Figure S7.

Back to article page