Figure 3

Fluorescence melting of the RNA duplexes composed with template strand and (a) primer A, (b) primer C, (c) primer G, and (d) primer U, respectively. The measurements were carried out in 200 nM each RNA duplex, 50 mM Tris–HCl (pH 8.0), 10 mM MgCl₂, and 5 mM DTT.