Table 2 Percentages of extended primers with NTPs at the first position next to the 3′ terminal of each primer in 0 or 20 wt% PEG200.

From: Dielectricity of a molecularly crowded solution accelerates NTP misincorporation during RNA-dependent RNA polymerization by T7 RNA polymerase

Substrate

Primer

Primer A

Primer C

Primer G (Matched)

Primer U

(-)PEG200

( +)PEG200

(−)PEG200

( +)PEG200

(−)PEG200

( +)PEG200

(−)PEG200

( +)PEG200

ATP

0a

0a

30 ± 2.2

39 ± 1.4

9.0 ± 2.2b

25 ± 8.1b

8.8 ± 0.6

13 ± 1.7

CTP

7.5 ± 1.2

18 ± 1.7

15 ± 1.7

30 ± 0.6

29 ± 11b

35 ± 13b

13 ± 0.9

25 ± 1.2

GTP

21 ± 7.0

28 ± 5.0

33 ± 5.2

56 ± 10

23 ± 12b

33 ± 20b

18 ± 1.1

34 ± 7.1

UTP (Matched)

8.4 ± 1.4

17 ± 3.4

12 ± 0.7

14 ± 1.5

50 ± 14b

37 ± 20b

16 ± 4.1

17 ± 4.1

  1. aNo bands for extended primers were detectable.
  2. bData were taken from the reference26.
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