Figure 2 | Scientific Reports

Figure 2

From: Silencing acetyl-CoA carboxylase A and sterol regulatory element-binding protein 1 genes through RNAi reduce serum and egg cholesterol in chicken

Figure 2

Southern blotting of transgenic birds. Lane 1: shRNA construct, Lanes 2–5: Transgenic birds of 1st generation, Lane6&7: Transgenic birds of 2nd generation; Lane8: Non-transgenic control bird; Lane9: Amplified product from shRNA plasmid construct acted as positive control for Southern blotting. Before running the gel, genomic DNA was digested with ApaI restriction enzyme. The digested sample was run on 0.8% agarose gel and was transferred to PVDF membrane. A 286 bp fragment located at the back bone of pENTR™/U6 vector was amplified from the shRNA construct and was used for preparation of probe. The probe was labelled with biotin-streptavidin conjugated to alkaline phosphatase and detected with NBT/BCIP for spot hybridization.

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