Table 1 Hatching performance of birds in transgenic and control groups during 1st generation.

From: Silencing acetyl-CoA carboxylase A and sterol regulatory element-binding protein 1 genes through RNAi reduce serum and egg cholesterol in chicken

Gene

Clone name

No. of eggs set in the hatcher

No. of fertile eggs detected upon candling

Fertility (%)

No. of chicks hatched

Hatchability on fertile egg set (%)

Hatchability on total egg set (%)

Positive transgenic chicks

Percentage of obtaining transgenic chicks (%)

ACACA

shRNA3

30

14

46.7

13

92.9

43.3

1

7.7

shRNA4

30

17

56.7

16

94.1

53.3

1

6.3

SREBP1

shRNA1

30

12

40.0

12

100.0

40.0

1

8.3

shRNA2

30

18

60.0

17

94.4

56.7

1

5.9

Control group

30

25

83.3

24

96.0

80.0

0

0

  1. Fertile eggs were incubated in the egg incubator and candling was done on 18th day to screen the fertile eggs. On 18th day, ggs were incubated in the hatcher and chicks were hatched on 21st day. Two shRNA clones each of ACACA and SREBP1 genes were transferred to the host through sperm mediated gene transfer and the transfected sperms were inseminated to the hens under treatment group while normal sperms were inseminated to the hens under control group. Fertile eggs from both treatment and control groups were collected for hatching to chicks.
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