Table 7 Progesterone and estrogen content in serum of adult transgenic and control birds.

From: Silencing acetyl-CoA carboxylase A and sterol regulatory element-binding protein 1 genes through RNAi reduce serum and egg cholesterol in chicken

Wing Band No of birds

Individual bird-wise progesterone and estrogen estimates

Overall transgenic and control group-wise progesterone and estrogen estimates and difference between transgenic and control groups during 1st generation

Molecule

Age (Weeks)

Generation

Ovulation cycle

Gender

Progesterone (ng/ml)

Estrogen (pg/ml)

Groups

Progesterone (ng/ml)

Estrogen (pg/ml)

First generation

1479

ACACA_shRNA4

51

1st

1–2 h after ovulation

Female

0.98

209.50

Control group

0.23 ± 0.07 a

204.40 ± 21.50

1445

ACACA_shRNA3

51

1st

1–2 h after ovulation

Female

0.76

286.40

ACACA transgenic group

0.87 ± 0.15 c

247.90 ± 54.30

        

P

0.0508

0.2414

1650

SREBP1_shRNA2

51

1st

1–2 h after ovulation

Female

0.55

182.80

Percent change in ACACA transgenic group (%)

278.20

21.20

1661

SREBP1_shRNA2

51

1st

1–2 h after ovulation

Female

0.66

244.70

SREBP1 transgenic group

0.61 ± 0.07 b

213.70 ± 43.70

        

P

0.0207

0.4152

1721

Control

51

1st

1–2 h after ovulation

Female

0.18

189.20

Percent change in SREBP1 transgenic group (%)

165.20

4.50

1728

Control

51

1st

1–2 h after ovulation

Female

0.28

219.70

   

Second generation

5330

ACACA_shRNA3

25

2nd

-

Male

0.16

86.26

   

4839

ACACA_shRNA3

25

2nd

1–2 h after ovulation

Female

0.44

138.16

   

5333

Control

25

2nd

-

Male

0.25

108.55

   
  1. The hormones were estimated during 1–2 h after ovulation at 51 weeks of age by chemiluminescence immunoassay to compare between transgenic and control groups.
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