Figure 1

(a) One frame from a movie of a sarcoma cell from the metastatic population T15 acquired using LSM 510 (Zeiss) equipped with Plan-Apochromat 63 × /1.4 Oil Ph3. The field size is 93 × 63 µm. (b, c) Micrographs of fixed sarcoma cells stained with rhodamine-phalloidin acquired as stacks using LSM 510 (Zeiss) are presented as maximum intensity projection with green pseudo-color. (b) K2 cells from the non-metastatic line feature conspicuous stress fibers. The field size is 154 × 184 µm. (c) A297 cells from the highly metastatic line, which have lost the stress fibers. The field size is 140 × 159 µm.