Figure 2
From: Addressing cancer invasion and cell motility with quantitative light microscopy
Images from a time-lapse sequence recording red and green fluorescence of pneumocytes type II and human non-small cell lung carcinoma A549 cells, respectively. (a) pneumocytes labeled red at time point 0 min; (b) A549 cells labeled green at time point 0 min; (c) overlay at time point 0 min; (d) pneumocytes labeled red at time point 210 min; (e) A549 cells labeled green at time point 210 min; (f) overlay at time point 210 min. The yellow arrow at the same position in each image points to an invasion even. The green A549 cell migrated towards the arrow first and subsequently invaded the confluent pneumocyte monolayer opening a hole. This created a black gap between the red pneumocyte cells, which were originally in close contact. We know from confocal microscopy that the invading cells attach to the substrate when displacing the pneumocytes. The presented field size is 434 × 434 µm.
