Figure 1 | Scientific Reports

Figure 1

From: Bacillus subtilis spores as delivery system for nasal Plasmodium falciparum circumsporozoite surface protein immunization in a murine model

Figure 1

Construction and confirmation of the expression of the rPfCSP. (A) A graphical representation of CSP. The region corresponding to the construction of the rPfCSP developed in this study is highlighted; (B) Amino acid sequence corresponding to rPfCSP and the expression plasmid in which it was inserted (pRSET A, Invitrogen); (C) SDS-PAGE analysis of recombinant protein, BLUeye Pre-stained ladder (Sigma-Aldrich) (lane M), and electrophoretic profile of rPfCSP elution (lane 1); (D) western blot confirming the rPfCSP antibodies recognition. Lane 1: anti-HisG monoclonal antibody recognition. Black, blue, red, and green arrows indicate ~ 120, 63, 33, and 25 kDa proteins, respectively. Development step was performed using BCIP/NBT chromogenic substrate from Western breeze kit (Invitrogen); Lane 2: anti-PfCSP monoclonal antibody (BEI Resources, cat. No. MRA-183A 2A10) recognition. Blue, red, and green arrows indicate 63, 33, and 25 kDa proteins, respectively. Development step was performed using 3,3'-diaminobenzidine (DAB, Sigma-Aldrich. cat. No. D7304).

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