Figure 4
Evaluation of the treatment on follicular activation and development. (a) Quiescent follicle (primordial stage) rates and (b) growing follicle (primary, secondary and antral stages) rates. (c) Proliferative rates of follicles following the treatment. Follicles with at least one granulosa cell stained were counted as positive and reported to the total number of follicles. (d) Representative micrographs of Ki67 positive stained follicles. NC, negative control (scale bar 20 μm). Data are presented as mean ± SD; n = 5/treatment/genotype; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
