Figure 5
Follicular activation assessment following co-administration of carboplatin and paclitaxel to Brip1+/− and Brip1+/+ mice. (a) Intensity of KIT LIGAND based on the signal of 20 randomly selected primordial and primary follicles per ovary (scale bar = 20 μm). (b) Illustration of the increasing KIT LIGAND staining intensity in primordial and primary follicles (scale bar = 20 μm). (c) Expression levels of Pten involved in follicular activation in the ovaries of Brip1+/− and Brip1+/+ mice. (d) Percentage of p-RPS6 positive follicles. (e) Representative micrographs of p-RPS6-positive primary and primordial follicles. NC, negative control; scale bar 20 μm. Data are presented as mean ± SD; n = 5/treatment/genotype; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
