Figure 6
Evaluation of apoptosis and DNA damage in the ovaries of Brip1+/− and Brip1+/+ mice. (a) Quantification of the degree of global apoptosis in the ovaries of treated mice. (b) Representative micrographs of nuclear labelling with Hoechst (blue) and TUNEL-positive (red) apoptotic cells from ovarian sections from mice treated with the combination of carboplatin and paclitaxel and the control group (scale bar = 200 μm). (c) Percentage of γH2AX-positive primordial follicles. (d) Representative micrographs of γH2AX-positive primordial follicles. NC, negative control; scale bar = 20 μm. Data are presented as mean ± SD; n = 5/treatment/genotype; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
