Figure 2 | Scientific Reports

Figure 2

From: M-CSF supports medullary erythropoiesis and erythroid iron demand following burn injury through its activity on homeostatic iron recycling

Figure 2

Effect of post burn M-CSF blockade on bone marrow cells. Bone marrow cells were harvested on day seven post injury from mice that received isotype or M-CSF neutralizing antibodies and analyzed using flow cytometry. (A) Gating of erythroid cells. (B) Erythroid cell populations plotted as % of marrow, n = 6/group. (C) CD71 MFI measured on the surface of erythroid populations E1 to E3, n = 6/group. (D) Gating and percentage of dead erythroblasts, n = 6/group. (E) Gating of myeloid cells after CD45+ pre-gate. (F) Myeloid populations plotted as % of marrow, n = 6/group. Data shown as box plots with whiskers at min and max. *p < 0.05 burn isotype versus sham isotype and #p < 0.05 burn anti-M-CSF versus burn isotype, ANOVA.

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