Figure 7

Exacerbation of iron restricted erythropoiesis caused by post burn M-CSF blockade is at least in part IL-6 dependent and associated with induction of hepcidin. Samples were harvested on day seven post injury from mice that received isotype or M-CSF neutralizing antibodies or from post burn day seven mice that received isotype, M-CSF neutralizing, or a combination of M-CSF and IL-6 neutralizing antibodies. (A) Pooled analysis of liver hepcidin expression relative to Hprt from three independent experiments for a total of n = 18/group. (B) Liver hepcidin expression relative to Hprt for n = 6/group. (C) Total non-heme iron levels in liver and spleen n = 5–6/group. (D) CD71 MFI of bone marrow erythroid populations E1 to E3 for n = 6/group. (E) ADVIA reticulocyte iron indices for n = 6/group. Panel (A) shown as dot plot with line at mean, *p < 0.05 sham isotype versus burn isotype and ^#p < 0.05 burn isotype versus burn anti-M-CSF, ANOVA. All other panels shown as box plots with whiskers at min and max, *p < 0.05 burn isotype versus burn anti-M-CSF and ^#p < 0.05 burn anti-M-CSF versus burn anti-M-CSF+ anti-IL-6.