Figure 5

Apoptosis induction by docetaxel and lovastatin in HGT-1 spheroids. (a) Red fluorescence photographs of HGT-1 spheroids at 0 h and 72 h of treatment with 5 nM docetaxel (D 5 nM), 12.5 µM lovastatin (L 12.5 µM) and 5 nM docetaxel + 12.5 µM lovastatin (D + L) in presence of Annexin V fluorescent reagent (Incucyte) captured with the Incucyte live imaging. (b) Annexin V binding on 6 days-old HGT-1 MCTS after 72 h of treatment with 5 nM docetaxel (D 5 nM), 12.5 µM lovastatin (L 12.5 µM) and 5 nM docetaxel + 12.5 µM lovastatin (D + L). The Annexin V reagent was added at the same time as the drugs. The results are shown as the mean ± SD of n = 3 independent experiments with four technical replicates in each. ns, p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001, one-way ANOVA followed by Tukey analysis.