Figure 6

Growth of bicellular MCTS. (a) Bright field photographs of 6 days-old monocellular spheroids formed from 500 or 250 HGT-1 cells and bi-cellular (250 CAF + 250 HGT-1) spheroids captured with the Incucyte live imaging. (b) Real-time growth monitoring (Area 10⁵ × µm²) was performed using the Incucyte live imaging. Monocellular MCTS generated from: (i) 250 HGT-1 cells is represented by white circles, (ii) 500 HGT-1 cells is represented by black X symbol and (iii) bicellular MCTS generated from 250 HGT-1 + 250 CAF is represented by X inside circles. The results are shown as the mean ± SD of n = 3 independent experiments with four technical replicates in each. *p ≤ 0.05; one-way ANOVA followed by Tukey analysis.