Figure 7
Representative electrophysiological analysis of primary nasal CF airway epithelia expanded using CRC conditions and differentiated in E-ALI. (A, B) CFhNE cells with the G551D/F508del genotype were expanded using CRC conditions to P4 and differentiated in E-ALI for 14 days. Shown are two representative traces demonstrating low levels of CFTR currents and modest responses to Vx-770 and Vx-809. (C, D) CFhNE cells with the W1282X/F508del genotype were expanded and differentiated as described above. Shown are two representative traces demonstrating low levels of CFTR currents and responses to Vx-770 and curcumin. FSK, Forskolin; inh172, CFTR channel inhibitor; Vx-770, Ivacaftor; Vx-809, Lumacaftor.
