Figure 1 | Scientific Reports

Figure 1

From: Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)

Figure 1

Metformin regulated expression of stress ligands and ICAM-I in leukemic cells. (A) Different AML cell lines OCI-AML-3 wtp53, HL-60 nullp53, NB4 mutp53 were treated with 2 mM metformin for 3 days and plasma membrane expression of the stress ligands MICA/B and ULBP1, the integrin ICAM-1, the myeloid marker CD33 and MHC class I HLA were analyzed by FACs analysis. (B) The different cell lines were treated as in (A) and the total number of cells and the percentage of alive cells were measured on the Muse Cell Analyzer. (C) OCI-AML3 cells were treated with different concentrations (0.0316; 0.1; 0.316; 1 and 2 mM) of metformin for 3 days, metformin was added every day and plasma membrane expression of the stress ligands MICA/B and ULBP1, the integrin ICAM-1 and the cell line marker CD33 were analyzed by FACs analysis. (D) Different MM cell lines MM1.S wtp53 and U266 mutp53 and the pro-monocytic myeloid leukemia cell U937 nullp53 were treated with 2 mM metformin for 3 days and the expression of the above described markers plus the MM cell line marker CD138 were analyzed by FACs. Data represent the Mean Fluorescence Intensity (MFI) levels compared to control, non-treated, cells. The bar graphs represent means ± SD of 3 independent experiments; *p < 0.05, **p < 0.01, ***p < 0.005 student t-test compare to control cells.

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