Figure 4

Normal development of E1 cells in Zfta^tm1/tm1 mice. (A–D) Wholemount preparations of the lateral walls of the LV were observed by SEM in WT (A) and Zfta^tm/tm (B) mice. Bar = 2.5 µm. The number (C; n = 40 E1 cells from 4 control mice, n = 41 E1 cells from 4 ZFTA^tm/tm mice; p = 0.80) and length (D; n = 48 cilia from 4 control mice, n = 48 cilia from 4 Zfta^tm/tm mice; p = 0.28) of E1 cells’ motile cilia were quantified and plotted. Data shown are the mean ± SEM. Each point on the graph is the number of cilia in an individual E1 cell (C) and the length of an individual cilium (D). N.S., not significant. (E–K) Wholemount preparations of the lateral walls of LV at P30–35 were stained with antibodies against γ-tubulin (green in E, F), GFAP (green in H, I), acetylated tubulin (green in J, K) and β-catenin (red in E, F, magenta in E, F, H–K) in the control (E, H, J) and Zfta^tm/tm (F, I, K) mice. Bars = 10 µm (H, I, L–Q) and 40 µm (I, K). (G) The number of BBs was quantified in the WT (n = 48 E1 cells from 3 mice) and Zfta^tm1/tm1 (n = 48 E1 cells from 3 mice, p = 0.50) E1 cells. Data shown are the mean ± SD. Each point on the graph is the number of BBs in an individual E cell. (L–Q) Coronal vibratome sections were stained with anti-αSMA (green in L, M), anti-FoxJ1 (magenta in L, M, P, Q), anti-CD24 antigen (green in N, O), anti-S100β (magenta in N, O) and anti-RFX1 (green in P, Q) antibodies in the control (L, N, P) and ZFTA^tm1/tm1 (M, O, Q) mice at P30–35. Bar = 10 µm. The white arrows in N and O indicate the CD24-immunoreactive motile cilia of E1 cells. The white arrows and arrowheads in P and Q indicate the RFX1/FoxJ1-double positive E1 cells and RFX1-positive parenchymal cells, respectively. LV, lateral ventricle; St., striatum.