Figure 5

Normal translational polarity in Zftatm/tm E1 cells. (A) Schematic drawing showing the BB patch angle and BB patch displacement. A vector (red arrow) was drawn from the centre of the apical surface to that of the BB patch. To calculate the BB patch displacement, the length of the vector was normalised with the length of the line extended to the edge of the apical surface (black line). (B) Quantification of the BB displacement. Data shown are the mean ± SD. Control; n = 87 E1 cells, 3 mice: Zftatm1/tm1; n = 86 E1 cells, 3 mice; p = 0.78. Each point on the graph is the BB displacement of an individual E1 cell. N.S., not significant. (C) Histogram showing the distribution of BB angles in the controls [white bars, circular standard deviation (CSD) = 57.19, n = 87 E1 cells, 3 mice] and Zftatm1/tm1 (CSD = 69.534, n = 86 E1 cells from 3 mice) E1 cells at P30–35. N.S., not significant (p > 0.1).