Figure 3

(A) A neighbor-joining phylogenetic tree was reconstructed to infer the relationship of mtCO1 sequences identified in the current study with the already known species of B. tabaci. Tree was rooted on the sequence of Bemisia tuberculata (AY057220) species and a total of 113 sequences were used in this analysis including 82 sequences identified in the current study and other reference sequences of some other species. The multiple sequences alignment was carried out by MUSCLE program in MEGA7 software³². Bootstrap method was used with 1000 replications and the percentage bootstrap value (greater than 50%) is shown on each breach. The 80 sequences identified in the current study made the group with Asia II 1 reference sequences (n = 3) and this group is collapsed at the top of the tree showing Asia II 1 (n = 82) whereas two sequences identified in the current study made the clade with Asia II 7 reference sequences. The isolates of the current study are labelled with red circle in the start of their mentioned name in the tree. (B) A neighbor-joining phylogenetic tree was reconstructed by using the current sequences and the reference sequences of only species identified in the current study and an out-group sequence mentioned above. This was included to show the relations among the sequences of the current study that was collapsed in (A).