Figure 1

Identification of isoaccepting tRNAs differentially released in extracellular vesicles of urine from kidney injury. (a and b) Interleaved scatter plots showing expression levels of the indicated RNA species, miRNA (a) and tRNA (b) in urinary extracellular vesicles from sham (blue) or injured (red) animals during the time course of renal ischemia/reperfusion injury (IRI). The states of injury and recovery were determined based on the levels of serum creatinine and urea nitrogen. The dataset used for this re-analysis was previously published in GEO database (GPL18694) and our publication¹⁴. All read counts were normalized using DEseq2 package. (c) A scatter plot showing normalized expression levels of isoacceptor tRNAs in urinary extracellular vesicles from sham (blue) and IRI (red) at the indicated days following renal ischemia/reperfusion injury. Alphabet letters denote the 21 genetically-encoded amino acids. All read counts were normalized using DEseq2 package.