Figure 8
Fibrotic remodeling of visceral adipose tissue in PDAC patients and an orthotopic implantation model appears to correlate with activin A. (A) Picro-Sirius Red staining of gonadal adipose tissue of sham control and S2-013 mPDAC. Arrows indicate areas of collagen deposition. (B) Picro-Sirius Red staining of visceral adipose tissue in PDAC patients with corresponding activin A levels in ng/ml. Arrows indicate areas of collagen deposition. (C) Area percentage of red pixels indicating collagen I/III deposition in gonadal adipose tissue from sham control and S2-013 mPDAC as measured by Image J. The average area percentage of red pixels from each animal was marked with a dot. (D) Area percentage of red pixels indicating collagen I/III deposition in visceral adipose tissue from PDAC patients with corresponding activin A levels in ng/ml. The areas of collagen I/III deposition were measured from several histological sections. 7 sections, 0.761 ng/ml; 13 sections, 2.434 ng/ml; 14 sections, 3.966 ng/ml; 11 sections, 4.160 ng/ml; 7 sections, 8.984 ng/ml; 11 sections, 14.362 ng/ml. Each dot indicates the area per section. (E) Immunofluorescence images with collagen I and fibronectin in visceral adipose tissue from patients with low and high serum activin A in ng/ml. Nuclei were stained with DAPI. Purple stars indicate adipocyte nuclei, while orange arrows indicate areas of signal. Scale bar = 50 µm for all microscope images. (F). Picro-Sirius Red staining of gonadal and subcutaneous white adipose tissue (WAT) obtained from C57BL/6 mice and cultured ex vivo with RPMI1640 (control), CM from KPC8069 cells, or CM from KPC8069 cells supplemented with FST288. Blue arrows indicate areas of collagen deposition.
