Table 5 Primer sequences and thermal conditions used for molecular detection of leptospira spp sheep and goates urine samples.
From: The role of small ruminants in the epidemiology of leptospirosis
Gene | Description | Sequence | Size | Cycling conditions | Reference |
|---|---|---|---|---|---|
16sr RNA | Forward (outer) | 5′-GGCGGCGCGTCTTAA ACATG-3′ | 525 | 95 °C—5 min; 30 × (95 °C —30 s, 55 °C—30 s, 72 °C —40 s); 72 °C—5 min | Dinparast et al.39 |
Reverse (outer) | 5′-GTCCGCCTACGCACCCTTTACG-3′ | ||||
16sr RNA | Forward (inner) | 5′-CAAGTCAAGCGGAGTAGCAA-3′ | 289 | 95 °C—5 min; 30 × (95 °C—30 s, 58 °C—30 s, 72 °C—30 s); 72 °C—5 min | |
Reverse (inner) | 5′-TAACCTGCTGCCTCCCGTA-3′ |
