Figure 2

Qualitative and Quantitative Descriptions of Microglia Morphotypes. The Bland-Altmann graphs show the difference between either IsoData (a) or Moments (b) CBCS cell counts and the manual counts on the y axis, against the average of IsoData or Moments and the manual counts. The dotted lines in blue show the average of differences. That in red show the average \(\pm\) 1.96 Standard Deviation. For (c), literature references^{4,10,12,16,30,31,32,33,34,35,36} were used to deduce qualitative descriptors of the cell bodies of each microglia morphotype. The unique descriptors per morphotype are in bold. Literature reference¹⁰ and own data were used to deduce the quantitative descriptors of the cell bodies of each microglia morphotype. The examples are the binarized images obtained from the AS to show the cell body images per microglia morphotype. The yellow circle has a diameter of 13.201 μm. \({F}_{max}\) = Feret’s Maximum Diameter; FDR Feret’s Diameter Ratio. (d) shows the shape of ramified and rod cell bodies to explain how \({F}_{max}\) may be an easier quantitative parameter to distinguish between both cell body morphotypes than area. The areas of the two shapes are very similar (53.500 vs 58.719μm²) whilst the \({F}_{max}\) of the ramified is less than half of that of the rod cell body (10.0001 vs 22.604 μm).