Figure 5 | Scientific Reports

Figure 5

From: Evaluation of stable reference genes for qPCR normalization in circadian studies related to lung inflammation and injury in mouse model

Figure 5

Bmal1 gene relative expression in HDM and PBS exposed mouse lung and data normalized with different reference genes. (A) Rn18s, (B) Rplp0, (C) Tbp, (D) Rplp0 + Hprt1 and (E) Actb. Line graphs showing the relative expression of the Bmal1 gene in both HDM versus PBS group. White and gray areas in the graph represents the light and dark cycle, respectively. Symbols † and # represent the statistical significance in temporal expression of Bmal1 in the PBS and HDM group respectively at the marked time point when compared to the baseline (ZT0). Data are shown as mean ± SEM (n = 4/group; † P < 0.05, †† P < 0.01, ††† P < 0.001, compared to ZT0 PBS; # P < 0.05, # # P < 0.01, # # # P < 0.001, compared to ZT0 HDM).

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